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KMID : 1134820210500080792
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Journal of the Korean Society of Food Science and Nutrition
2021 Volume.50 No. 8 p.792 ~ p.798
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Evaluation of Antioxidant and Antimicrobial Activity of Buah Merah (Pandenus conoideus) by Solvent Fractionation
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Kim Min-Zy
Kim Kyoung-Hee
Jang Hyun
Yook Hong-Sun
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Abstract
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Buah merah is a plant native to Papua, Indonesia, and is rich in antioxidants. In this study, the extraction method of the antioxidant content of the buah merah was optimized. The phenol content was found to be 0.6 to 2.0 GAE mg/g, and the activity was the highest in dichloromethane. Total flavonoid content was found to be 0.1 to 2.7 CE mg/g and showed the highest activity in dichloromethane. The ferric reducing ability of plasma, measurement was found to be 5.1 to 44.8 mM/g, and the highest value was seen in dichloromethane. ABTS radical scavenging activity showed an IC?? value of 3.2∼27.7 mg/mL, and the lowest value was 1.8 mg/mL in dichloromethane. DPPH radical scavenging activity showed an IC50 value of 1.6∼48.41 mg/mL, and the lowest value was 1.6 mg/mL in dichloromethane. It was found that each fraction showed antimicrobial activity in the experiment. The hexane fraction showed the highest antimicrobial activity against E. cloacae and B. cereus, and the dichloromethane fraction showed the highest antimicrobial activity against S. aureus and E. coli. The butanol fraction showed the highest activity against S. aureus, B. cereus, and S. enterica, and the water fraction showed the highest activity against S. enterica and S. aureus. Based on all these results, it was found that the dichloromethane was the most active and effective fraction of buah merah.
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KEYWORD
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Buah merah (Pandenus conoideus), antioxidant activity, solvent fraction, antimicrobial activity
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